Part:BBa_K619896:Design

Pcon lox gent double terminator lox

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 811
  Illegal BamHI site found at 1300
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 167
  

Design Notes

indicated under sources.

Source

The Pcon comes from BBa_J23106, the gentamycin resistance flanked by lox sites was a gift from Dr. Joel Griffitts and was amplified from pJG181.